Diagnosis, management and control of emerging infectious diseases like COVID-19 can be a dilemma in resource-limited settings. Official case counts suggest Africa has not seen the expected burden of COVID-19 as predicted by international health agencies, and the proportion of asymptomatic patients, disease severity, and mortality burden differ significantly in Africa from what has been observed elsewhere. Testing for SARS-CoV-2 was extremely limited early in the pandemic and likely led to under-reporting of cases leaving important gaps in our understanding of transmission and disease characteristics in the African context. We aimed to train medical facilitators to use PCR and RT PCR techniques to identify SARS-CoV-2 virus for rapid and specific diagnsotic in Cameroon.
Specifically we will train on :
1- General Molecular Biology Protocols
a- Sample collectio and storage
b- Genomic DNA Isolation
c- Protein extraction
d- RNA extraction
e- cDNA preparation/ RTPCR
f- Gel electrophoresis
2- PCR
a- Allell specific PCR
b- Nested/ Hemi nested PCR
c- Real time PCR
d- PCR-RFLP
3- DNA Methyllation study
4- Immuno Blotting
Period :
We will spend three days to train health workers on molecular biology techniques from 25 October to 27 October 2021
Place of training:
The train will take place at Ecotourism Park of Cameroon
Training program :
Time DAY 1 8H:00 – 9H:00 Registration and setting Up 09H00- 09H:30 Introductive Talk 09H30 – 10H:00 PICTURE AND COFFEE BREAK 10H:00 – 12H:00 SESSION 1: General molecular Biology Protocols 12H:00 -13H00 BREAK WITH LUNCH 13H:00- 15H:00 SESSION 2: PCR techniques 15H:00 – 15H15 Coffee break 15H:15 – 16H45 SESSION3: Practicals
DAY 2 08H30- 09H:30 SESSION 4: DNA Methyllation study 09H30 – 10H:00 PICTURE AND COFFEE BREAK 10H:00 – 12H:00 SESSION 4 CON’t: DNA meethylation study 12H:00 -13H00 BREAK WITH LUNCH 13H:00- 15H:00 SESSION 4: tittle practicals 15H:00 – 15H15 Coffee break Day 3 08H30- 09H:30 SESSION : Immuno blotting 09H30 – 10H:00 PICTURE AND COFFEE BREAK 10H:00 – 12H:00 SESSION 4 CON’t: practical 12H:00 -13H00 BREAK WITH LUNCH 13H:00- 15H:00 SESSION 5: Practical
